HLA-DRB5 Antibody
| Code | Size | Price |
|---|
| PSI-64-133-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
HLA class II histocompatibility antigen, DR beta 5 chain, DR beta-5, DR2-beta-2, Dw2, MHC class II antigen DRB5, HLA-DRB5 {ECO:0000312|EMBL:CAI180791}
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
HLA-DRB5 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB5 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Background References:
- Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)
- Barrett, J.C., et al. Nat. Genet. 41(12):1330-1334(2009)
- Montano-Loza, A.J., et al. Liver Int. 29(10):1543-1551(2009)
- Lincoln, M.R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(18):7542-7547(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HLA-DRB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the Central region of human HLA-DRB5.
NCBI Gene ID #:
3127
NCBI Official Name:
HLA class II histocompatibility antigen, DR beta 5 chain
NCBI Official Symbol:
HLA-DRB5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
30 kDa
Protein Accession #:
Q30154
Protein GI Number:
74754558
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q30154
User NOte:
Optimal dilutions for each application to be determined by the researcher.