LYZ Antibody

Contact us
ProSci
Product Code: PSI-61-062
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-062-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: WiDr whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: Human spleen lysate Lysates/proteins at 20 ug per lane.
2 / 5
Immunohistochemical analysis of paraffin-embedded H. small intestine section using LYZ Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
3 / 5
4 / 5
Immunohistochemical analysis of paraffin-embedded M. small intestine section using LYZ Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
5 / 5
Western blot analysis of lysates from HepG2, THP-1, U-937, WiDr cell line and human spleen(from left to right), using LYZ Antibody at 1:1000 at each lane.
❮❯

Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: WiDr whole cell lysate Lane 4: HL-60 whole cell lysate Lane 5: Human spleen lysate Lysates/proteins at 20 ug per lane.
Immunohistochemical analysis of paraffin-embedded H. small intestine section using LYZ Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded M. small intestine section using LYZ Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from HepG2, THP-1, U-937, WiDr cell line and human spleen(from left to right), using LYZ Antibody at 1:1000 at each lane.

Documents

Further Information

Additional Names:
Lysozyme C,4-beta-N-acetylmuramidase C, LYZ, LZM
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:25
Background:
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents.
Background References:
  • Castanon M.J.,et al.Gene 66:223-234(1988).
  • Chung L.P.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:6227-6231(1988).
  • Yoshimura K.,et al.Biochem. Biophys. Res. Commun. 150:794-801(1988).
  • Peters C.W.B.,et al.Eur. J. Biochem. 182:507-516(1989).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LYZ antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 119-154 amino acids from the C-terminal region of human LYZ.
NCBI Gene ID #:
4069
NCBI Official Name:
Lysozyme C
NCBI Official Symbol:
LYZ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
17 kDa
Protein Accession #:
P61626
Protein GI Number:
48428995
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P61626
User NOte:
Optimal dilutions for each application to be determined by the researcher.