CTGF Antibody

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ProSci
Product Code: PSI-63-197
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-197-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CTGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western Blot at 1:1000 dilution Lane 1: A549 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with CTGF antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Connective tissue growth factor, CCN family member 2, Hypertrophic chondrocyte-specific protein 24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8, CTGF, CCN2, HCS24, IGFBP8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Connective tissue growth factor (CTGF), a member of the CCN family of secreted matricellular proteins, regulates fibrosis, angiogenesis, cell proliferation, apoptosis, tumor growth, and metastasis.
Background References:
  • Li,M.H., Mol. Cancer Res.(10), 1649-1656 (2008)
  • Bogatkevich,G.S., Am. J. Physiol. Lung Cell Mol. Physiol. 295 (4), L603-L611 (2008)
  • Zhang,P., Tohoku J. Exp. Med. 215 (3), 199-206 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Pig, Bovine
Immunogen:
This CTGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-349 amino acids from the C-terminal region of human CTGF.
NCBI Gene ID #:
1490
NCBI Official Name:
Connective tissue growth factor
NCBI Official Symbol:
CTGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
P29279
Protein GI Number:
116241320
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology
Swissprot #:
P29279
User NOte:
Optimal dilutions for each application to be determined by the researcher.