IL25 Antibody

ProSci
Product Code: PSI-57-895
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-895-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).

Further Information

Additional Names:
Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.
Background References:
  • Xu, G., et al. Allergy 65(5):581-589(2010)
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  • Goswami, S., et al. Nat. Immunol. 10(5):496-503(2009)
  • Caruso, R., et al. Blood 113(15):3512-3519(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25.
NCBI Gene ID #:
64806
NCBI Official Name:
Interleukin-25
NCBI Official Symbol:
IL25
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q9H293
Protein GI Number:
20138730
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q9H293
User NOte:
Optimal dilutions for each application to be determined by the researcher.