HMHA1 Antibody

ProSci
Product Code: PSI-62-570
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-570-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of HMHA1 Antibody in A2058 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lymph reacted with HMHA1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of HMHA1 Antibody in A2058 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lymph reacted with HMHA1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Minor histocompatibility protein HA-1, Minor histocompatibility antigen HA-1, mHag HA-1, HMHA1, KIAA0223
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The minor histocompatibility antigen (mHags), HA-1 is a immunogenic alloantigen shown to be responsible for graft-versus-host disease (GVHD) in HLA-identical bone marrow transplantation. The antigen has two known alleles resulting in a single amino acid polymorphism. The HA-1H allele encodes histidine, whereas the HA-1R allele encodes arginine.
Background References:
  • Gillespie,G.,et.al., Hematol. J.(6), 403-410 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This HMHA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 877-903 amino acids from the Central region of human HMHA1.
NCBI Gene ID #:
23526
NCBI Official Name:
Minor histocompatibility protein HA-1
NCBI Official Symbol:
HMHA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
125 kDa
Protein Accession #:
Q92619
Protein GI Number:
187471158
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
Q92619
User NOte:
Optimal dilutions for each application to be determined by the researcher.