CD2BP2 Antibody

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ProSci
Product Code: PSI-55-522
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-522-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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CD2BP2 antibodyimmunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD2BP2 antibodyfor immunohistochemistry.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
CD2BP2 antibodyimmunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD2BP2 antibodyfor immunohistochemistry.

Documents

Further Information

Additional Names:
CD2 antigen cytoplasmic tail-binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 tail-binding protein 2, U5 snRNP 52K protein, U5-52K, CD2BP2, KIAA1178
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a bi-functional protein. In the cytoplasm, the encoded protein binds the cytoplasmic tail of human surface antigen CD2 via its C-terminal GYF domain, and regulate CD2-triggered T lymphocyte activation. In the nucleus, this protein is a component of the U5 small nuclear ribonucleoprotein complex and is involved in RNA splicing. A pseudogene has been identified on chromosome 7. Alternative splicing results in multiple transcript variants but their biological validity has not been determined.
Background References:
  • Heinze, M., et al. Int. Immunol. 19(11):1313-1318(2007)
  • Nielsen, T.K., et al. J. Mol. Biol. 369(4):902-908(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This CD2BP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-52 amino acids from the N-terminal region of human CD2BP2.
NCBI Gene ID #:
10421
NCBI Official Name:
CD2 antigen cytoplasmic tail-binding protein 2
NCBI Official Symbol:
CD2BP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
O95400
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
O95400
User NOte:
Optimal dilutions for each application to be determined by the researcher.