HLA-G Antibody

Product Code: PSI-63-713

Supplier: ProSci

Code	Size	Price

PSI-63-713-400ul

400ul

£662.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species: Human

Applications:

Fluorescence-activated cell sorting (FACS)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Raji whole cell lysate Lane 3: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.

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Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody ) at 1:1000 at each lane.

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Western blot analysis of HLA-G using rabbit polyclonal HLA-G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.

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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Documents

Further Information

Additional Names:

HLA class I histocompatibility antigen, alpha chain G, HLA G antigen, MHC class I antigen G, HLA-G, HLA-60, HLAG

Application Note:

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50

Background:

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

Background References:

Walpole,N.G., et.al., J. Mol. Biol. 397 (2), 467-480 (2010)
Tian,W., et.sl., Tissue Antigens 75 (3), 227-234 (2010)

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.

NCBI Gene ID #:

3135

NCBI Official Name:

HLA class I histocompatibility antigen, alpha chain G

NCBI Official Symbol:

HLA-G

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

38 kDa

Protein Accession #:

P17693

Protein GI Number:

122132

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Immunology

Swissprot #:

P17693

User NOte:

Optimal dilutions for each application to be determined by the researcher.