SPI1 Antibody

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ProSci
Product Code: PSI-58-037
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-037-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.
2 / 3
Western blot analysis in WiDr cell line lysates (35ug/lane).
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Fluorescent image of NIH/3T3 cell stained with SPI1 Antibody .NIH/3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SPI1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). SPI1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in WiDr cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Transcription factor PU1, 31 kDa-transforming protein, SPI1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Background References:
  • Rimmele, P., et al. Cancer Res. 70(17):6757-6766(2010)
  • Bonadies, N., et al. Oncogene 29(7):1062-1072(2010)
  • Bonadies, N., et al. Blood 115(2):331-334(2010)
  • Desai, S., et al. J. Immunol. 183(9):5778-5787(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Pig, Rat
Immunogen:
This SPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 217-246 amino acids from the C-terminal region of human SPI1.
NCBI Gene ID #:
6688
NCBI Official Name:
Transcription factor PU.1
NCBI Official Symbol:
SPI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
P17947
Protein GI Number:
60415923
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P17947
User NOte:
Optimal dilutions for each application to be determined by the researcher.