KLRC1 Antibody

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ProSci
Product Code: PSI-63-556
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-556-200ul200ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
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Western blot analysis of KLRC1 Antibody in Jurkat cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Overlay histogram showing Jurkat cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysates Lane 2: K562 whole cell lysates Lysates/proteins at 20 ug per lane.
Western blot analysis of KLRC1 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human kidney carcinoma with KLRC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
NKG2-A/NKG2-B type II integral membrane protein, CD159 antigen-like family member A, NK cell receptor A, NKG2-A/B-activating NK receptor, CD159a, KLRC1, NKG2A
Application Note:
For IF starting dilution is: 1:25

For FACS starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. This protein belongs to the killer cell lectin-like receptor family, also called NKG2 family, which is a group of transmembrane proteins preferentially expressed in NK cells. This family of proteins is characterized by the type II membrane orientation and the presence of a C-type lectin domain. This protein forms a complex with another family member, KLRD1/CD94, and has been implicated in the recognition of the MHC class I HLA-E molecules in NK cells.
Background References:
  • Brooks,A.G., et.al., J. Exp. Med. 185 (4), 795-800 (1997)
  • Plougastel,B., et.al., Immunogenetics 44 (4), 286-291 (1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KLRC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 180-206 amino acids from the C-terminal region of human KLRC1.
NCBI Gene ID #:
3821
NCBI Official Name:
NKG2-A/NKG2-B type II integral membrane protein
NCBI Official Symbol:
KLRC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P26715
Protein GI Number:
317373399
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P26715
User NOte:
Optimal dilutions for each application to be determined by the researcher.