CD46 Antibody

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ProSci
Product Code: PSI-61-663
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-663-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from Jurkat,SK-BR-3 cell line (from left to right),using CD46 Antibody .AP4785b was diluted at 1:1000 at each lane.
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CD46 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Confocal immunofluorescent analysis of CD46 Antibody with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysates from Jurkat,SK-BR-3 cell line (from left to right),using CD46 Antibody .AP4785b was diluted at 1:1000 at each lane.
CD46 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of CD46 Antibody with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Membrane cofactor protein, TLX, Trophoblast leukocyte common antigen, CD46, CD46, MCP, MIC10
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
CD46 is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. This gene is found in a cluster on chromosome 1q32 with other genes encoding structural components of the complement system.
Background References:
  • Weyand, N.J., et al. J. Immunol. 184(2):694-701(2010)
  • Santiago, C., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 66 (PT 1), 91-94 (2010)
  • Sullivan, M., et al. Ann. Hum. Genet. 74(1):17-26(2010)
  • Lee, S.W., et al. J. Cell Biol. 156(6):951-957(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD46 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-343 amino acids from the C-terminal region of human CD46.
NCBI Gene ID #:
4179
NCBI Official Name:
Membrane cofactor protein
NCBI Official Symbol:
CD46
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
P15529
Protein GI Number:
41019474
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology
Swissprot #:
P15529
User NOte:
Optimal dilutions for each application to be determined by the researcher.