APOBEC3F Antibody

ProSci
Product Code: PSI-63-821
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-821-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of APOBEC3F Antibody in K562 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of APOBEC3F Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
DNA dC->dU-editing enzyme APOBEC-3F, 354-, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F, A3F, APOBEC3F
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.
Background References:
  • Khatua,A.K., et.al., Virology 400 (1), 68-75 (2010)
  • Koning,F.A., et.al., J. Virol. 83 (18), 9474-9485 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APOBEC3F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human APOBEC3F.
NCBI Gene ID #:
200316
NCBI Official Name:
DNA dC->dU-editing enzyme APOBEC-3F
NCBI Official Symbol:
APOBEC3F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
Q8IUX4
Protein GI Number:
161784334
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,
Swissprot #:
Q8IUX4
User NOte:
Optimal dilutions for each application to be determined by the researcher.