NRP1 Antibody

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ProSci
Product Code: PSI-63-766
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-766-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of NRP1 Antibody in MDA-MB435 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NRP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of NRP1 Antibody in MDA-MB435 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NRP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, NRP1, NRP, VEGF165R
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.
Background References:
  • Hong,J.M., et.al., Exp. Mol. Med. (2010) In press Joslyn,G., et.al., Alcohol. Clin. Exp. Res. (2010) In press
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-750 amino acids from the C-terminal region of human NRP1.
NCBI Gene ID #:
8829
NCBI Official Name:
Neuropilin-1
NCBI Official Symbol:
NRP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
O14786
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Neuroscience
Swissprot #:
O14786
User NOte:
Optimal dilutions for each application to be determined by the researcher.