THY1 Antibody

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ProSci
Product Code: PSI-61-007
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-007-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-THY1 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate.
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Antibody is used in Western blot to detect THY1 in HL60 cell lysate.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-THY1 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide (BP2050a) in T47D cell line lysate.
Antibody is used in Western blot to detect THY1 in HL60 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
THY1 (CD90) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain.
Background References:
  • Koumas, L., et al., Am. J. Pathol. 163(4):1291-1300 (2003). Abeysinghe, H.R., et al., Cancer Genet. Cytogenet. 143(2):125-132 (2003). Saalbach, A., et al., Microvasc. Res. 64(1):86-93 (2002). Henniker, A.J., J. Biol. Regul. Homeost. Agents 15(4):392-393 (2001). Ye, Z., et al., Biochem. Biophys. Res. Commun. 275(1):223-227 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This THY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human THY1.
NCBI Gene ID #:
7070
NCBI Official Name:
Thy-1 membrane glycoprotein
NCBI Official Symbol:
THY1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
18 kDa
Protein Accession #:
P04216
Protein GI Number:
20455514
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Neuroscience,Signal Transduction
Swissprot #:
P04216
User NOte:
Optimal dilutions for each application to be determined by the researcher.