CD69 Antibody

ProSci
Product Code: PSI-61-918
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-918-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in HL-60 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in HL-60 cell line lysates (35ug/lane).
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69, CD69, CLEC2C
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants.
Background References:
  • Davila, S., et al. Genes Immun. (2010) In press :
  • Kolenko, P., et al. Acta Crystallogr. Sect.Struct. Biol. Cryst. Commun. 65 (PT 12), 1258-1260 (2009) :
  • Hu, M., et al. J. Mol. Recognit. 22(6):516-520(2009)
  • Radstake, T.R., et al. PLoS ONE(6), E5981 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CD69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human CD69.
NCBI Gene ID #:
969
NCBI Official Name:
Early activation antigen CD69
NCBI Official Symbol:
CD69
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
Q07108
Protein GI Number:
584906
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
Q07108
User NOte:
Optimal dilutions for each application to be determined by the researcher.