ICAM1 Antibody

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ProSci
Product Code: PSI-63-572
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-572-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of ICAM1 using rabbit polyclonal ICAM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ICAM1 gene (Lane 2).
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Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of ICAM1 using rabbit polyclonal ICAM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ICAM1 gene (Lane 2).
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Intercellular adhesion molecule 1, ICAM-1, Major group rhinovirus receptor, CD54, ICAM1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
ICAM1 is a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
Background References:
  • Denkers,I.A., et.al., Leuk. Res. 16 (5), 469-474 (1992)
  • Rossler,K., et.al., J. Neurosci. Res. 31 (2), 365-374 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ICAM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 488-514 amino acids from the C-terminal region of human ICAM1.
NCBI Gene ID #:
3383
NCBI Official Name:
Intercellular adhesion molecule 1
NCBI Official Symbol:
ICAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P05362
Protein GI Number:
68067956
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
P05362
User NOte:
Optimal dilutions for each application to be determined by the researcher.