CD28 Antibody

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ProSci
Product Code: PSI-62-568
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-568-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CD28 Antibody in MDA-MB231 cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of CD28 Antibody with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of CD28 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of CD28 Antibody with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Background References:
  • Zocchi,M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rb
Immunogen:
This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28.
NCBI Gene ID #:
940
NCBI Official Name:
T-cell-specific surface glycoprotein CD28
NCBI Official Symbol:
CD28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
P10747
Protein GI Number:
115973
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P10747
User NOte:
Optimal dilutions for each application to be determined by the researcher.