IL1RN Antibody

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ProSci
Product Code: PSI-62-283
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-283-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:4000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in 293,Hela cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of IL1RN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:4000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in 293,Hela cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of IL1RN Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, Anakinra, IL1RN, IL1F3, IL1RA
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
The protein is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of its gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer.
Background References:
  • Kumar,S., Biochem. Biophys. Res. Commun. 387 (3), 456-460 (2009) Rechcinski,T., Kardiol Pol 67 (6), 601-610 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL1RN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-177 amino acids from the C-terminal region of human IL1RN.
NCBI Gene ID #:
3557
NCBI Official Name:
Interleukin-1 receptor antagonist protein
NCBI Official Symbol:
IL1RN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P18510
Protein GI Number:
124312
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
P18510
User NOte:
Optimal dilutions for each application to be determined by the researcher.