NFATC1 Antibody

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ProSci
Product Code: PSI-57-339
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-339-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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NFATC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
NFATC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Nuclear factor of activated T-cells, cytoplasmic 1, NF-ATc1, NFATc1, NFAT transcription complex cytosolic component, NF-ATc, NFATc, NFATC1, NFAT2, NFATC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
Background References:
  • Liu, Y.J., et al. Obesity (Silver Spring) 18(12):2339-2346(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Chen, C., et al. Biocell 34(2):57-63(2010)
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NFATC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 898-927 amino acids from the C-terminal region of human NFATC1.
NCBI Gene ID #:
4772
NCBI Official Name:
Nuclear factor of activated T-cells, cytoplasmic 1
NCBI Official Symbol:
NFATC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
101 kDa
Protein Accession #:
O95644
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
O95644
User NOte:
Optimal dilutions for each application to be determined by the researcher.