SPN/CD43 Antibody

ProSci
Product Code: PSI-57-467
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-467-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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SPN/CD43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Jurkat cell line lysates (35ug/lane).
SPN/CD43 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Leukosialin, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43, SPN, CD43
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Sialophorin (leukosialin) is a major sialoglycoprotein on the surface of human T lymphocytes, monocytes, granulocytes, and some B lymphocytes, which appears to be important for immune function and may be part of a physiologic ligand-receptor complex involved in T-cell activation.
Background References:
  • Urano-Tashiro, Y., et al. Infect. Immun. 76(10):4686-4691(2008)
  • Mambole, A., et al. J. Biol. Chem. 283(35):23627-23635(2008)
  • Seethala, R.R., et al. Appl. Immunohistochem. Mol. Morphol. 16(2):165-172(2008)
  • Khunkaewla, P., et al. Mol. Immunol. 45(6):1703-1711(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SPN/CD43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-257 amino acids from the Central region of human SPN/CD43.
NCBI Gene ID #:
101929889, 6693
NCBI Official Name:
Leukosialin
NCBI Official Symbol:
SPN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
P16150
Protein GI Number:
126213
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P16150
User NOte:
Optimal dilutions for each application to be determined by the researcher.