LSP1 Antibody

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ProSci
Product Code: PSI-61-932
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-932-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:8000 dilution Lane 1: Daudi whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, pp52, Lymphocyte-specific antigen WP34, LSP1, WP34
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
LSP1 is an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms.
Background References:
  • Davila, S., et al. Genes Immun. (2010)
  • Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
  • Bhatti, P., et al. Radiat. Res. 173(2):214-224(2010)
  • Tamimi, R.M., et al. Cancer Causes Control (2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LSP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 108-134 amino acids from the Central region of human LSP1.
NCBI Gene ID #:
4046
NCBI Official Name:
Lymphocyte-specific protein 1
NCBI Official Symbol:
LSP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P33241
Protein GI Number:
462553
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P33241
User NOte:
Optimal dilutions for each application to be determined by the researcher.