FCN3 Antibody

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ProSci
Product Code: PSI-56-507
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-507-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:500 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:500 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Ficolin-3, Collagen/fibrinogen domain-containing lectin 3 p35, Collagen/fibrinogen domain-containing protein 3, Hakata antigen, FCN3, FCNH, HAKA1
Application Note:
For WB starting dilution is: 1:500

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum, there are two types of ficolins, both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein, which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus, has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants, each encoding a distinct isoform, have been identified.
Background References:
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  • Andersen, T., et al. J. Rheumatol. 36(4):757-759(2009)
  • Ruskamp, J.M., et al. Clin. Exp. Immunol. 155(3):433-440(2009)
  • Lacroix, M., et al. J. Immunol. 182(1):456-465(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FCN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 amino acids from the C-terminal region of human FCN3.
NCBI Gene ID #:
8547
NCBI Official Name:
Ficolin-3
NCBI Official Symbol:
FCN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
O75636
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
O75636
User NOte:
Optimal dilutions for each application to be determined by the researcher.