HCK Antibody
| Code | Size | Price |
|---|
| PSI-63-162-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck, HCK
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Background:
HCK is a member of the Src
family of tyrosine kinases. This protein is primarily hemopoietic,
particularly in cells of the myeloid and B-lymphoid lineages. It
may help couple the Fc receptor to the activation of the
respiratory burst. In addition, it may play a role in neutrophil
migration and in the degranulation of neutrophils. Multiple
isoforms with different subcellular distributions are produced due
to both alternative splicing and the use of alternative translation
initiation codons, including a non-AUG (CUG) codon. [provided by
RefSeq].
Background References:
- Hassan, R., et al. J. Cell. Physiol. 221(2):458-468(2009)
- Kennah, E., et al. Blood 113(19):4646-4655(2009)
- Voss, M., et al. BMC Immunol. 10, 53 (2009) :
- Rikova, K., et al. Cell 131(6):1190-1203(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat, Monkey
Immunogen:
This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from the N-terminal region of human HCK.
NCBI Gene ID #:
3055
NCBI Official Name:
Tyrosine-protein kinase HCK
NCBI Official Symbol:
HCK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P08631
Protein GI Number:
20141296
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P08631
User NOte:
Optimal dilutions for each application to be determined by the researcher.