MNDA Antibody

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ProSci
Product Code: PSI-55-731
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-731-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of MNDA Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in CEM cell line lysate
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MNDA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of MNDA Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in CEM cell line lysate
MNDA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Myeloid cell nuclear differentiation antigen, MNDA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The myeloid cell nuclear differentiation antigen (MNDA) is detected only in nuclei of cells of the granulocyte-monocyte lineage. A 200-amino acid region of human MNDA is strikingly similar to a region in the proteins encoded by a family of interferon-inducible mouse genes, designated Ifi-201, Ifi-202, and Ifi-203, that are not regulated in a cell- or tissue-specific fashion. The 1.8-kb MNDA mRNA, which contains an interferon-stimulated response element in the 5-prime untranslated region, was significantly upregulated in human monocytes exposed to interferon alpha. MNDA is located within 2,200 kb of FCER1A, APCS, CRP, and SPTA1. In its pattern of expression and/or regulation, MNDA resembles IFI16, suggesting that these genes participate in blood cell-specific responses to interferons.
Background References:
  • Kimkong, I., et al. J. Rheumatol. 37(7):1544-1547(2010)
  • Kanellis, G., et al. Leukemia 23(10):1847-1857(2009)
  • Joshi, A.D., et al. Clin. Cancer Res. 13 (18 PT 1), 5295-5304 (2007) :
  • Briggs, R.C., et al. Cancer Res. 66(9):4645-4651(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MNDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-53 amino acids from the N-terminal region of human MNDA.
NCBI Gene ID #:
4332
NCBI Official Name:
Myeloid cell nuclear differentiation antigen
NCBI Official Symbol:
MNDA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
P41218
Protein GI Number:
730038
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Immunology,Signal Transduction
Swissprot #:
P41218
User NOte:
Optimal dilutions for each application to be determined by the researcher.