LRG1 Antibody

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ProSci
Product Code: PSI-62-414
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-414-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of LRG1 antibody in 293 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with LRG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of LRG1 using rabbit polyclonal LRG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LRG1 gene.
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Western blot analysis of LRG1 antibody in 293 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with LRG1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of LRG1 using rabbit polyclonal LRG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LRG1 gene.

Documents

Further Information

Additional Names:
Leucine-rich alpha-2-glycoprotein, LRG, LRG1, LRG
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The leucine-rich repeat (LRR) family of proteins, including LRG1, have been shown to be involved in protein-protein interaction, signal transduction, and cell adhesion and development. LRG1 is expressed during granulocyte differentiation.
Background References:
  • Shirai,R., Biochem. Biophys. Res. Commun. 382 (4), 776-779 (2009)
  • Gillott,D.J., Fertil. Steril. 90 (3), 761-768 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-223 amino acids from the Central region of human LRG1.
NCBI Gene ID #:
116844
NCBI Official Name:
Leucine-rich alpha-2-glycoprotein
NCBI Official Symbol:
LRG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
P02750
Protein GI Number:
23503037
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
P02750
User NOte:
Optimal dilutions for each application to be determined by the researcher.