HPGD Antibody

ProSci
Product Code: PSI-62-470
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-470-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of HPGD Antibody in MDA-MB231 cell line lysates (35ug/lane)
3 / 4
4 / 4
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:2000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of HPGD Antibody in MDA-MB231 cell line lysates (35ug/lane)
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, Prostaglandin dehydrogenase 1, HPGD, PGDH1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
HPGD is a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. This protein is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation.
Background References:
  • Thill,M., et.al., Anticancer Res. 29 (9), 3619-3625 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This HPGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the C-terminal region of human HPGD.
NCBI Gene ID #:
3248
NCBI Official Name:
15-hydroxyprostaglandin dehydrogenase [NAD(+)]
NCBI Official Symbol:
HPGD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
P15428
Protein GI Number:
129889
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
P15428
User NOte:
Optimal dilutions for each application to be determined by the researcher.