PI3KCD Antibody

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ProSci
Product Code: PSI-63-347
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-347-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
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Western blot analysis of anti-PI3KCD Antibody Pab (AP8020d) in 293 cell line lysates.
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Fluorescent image of Hela cell stained with PI3KCD Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PI3KCD primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-PI3KCD Antibody Pab (AP8020d) in 293 cell line lysates.

Documents

Further Information

Additional Names:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform, PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha , p110-beta, and p110-gamma. p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins and with GTP-bound Ras.
Background References:
  • Khan, N.A., et al., J. Neurovirol. 9(6):584-593 (2003).
  • Sadhu, C., et al., J. Immunol. 170(5):2647-2654 (2003).
  • Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002).
  • Cook, J.A., et al., J. Immunol. 169(1):254-260 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PI3KCD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-114 amino acids from the N-terminal region of human PI3KCD.
NCBI Gene ID #:
5293
NCBI Official Name:
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
NCBI Official Symbol:
PIK3CD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
119 kDa
Protein Accession #:
O00329
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
O00329
User NOte:
Optimal dilutions for each application to be determined by the researcher.