M-CSF Antibody

Contact us
ProSci
Product Code: PSI-62-446
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-446-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody at 1:1000 at each lane.
2 / 4
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
3 / 4
4 / 4
M-CSF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
❮❯

Western blot analysis of lysate from human placenta tissue lysate, using M-CSF Antibody at 1:1000 at each lane.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
M-CSF Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim, Processed macrophage colony-stimulating factor 1, CSF1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50
Background:
CSF1 is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. This protein may be involved in development of the placenta.
Background References:
  • Lee,M.S., et.al.,J. Immunol. 183 (5), 3390-3399 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This M-CSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-257 amino acids from the Central region of human M-CSF.
NCBI Gene ID #:
1435
NCBI Official Name:
Macrophage colony-stimulating factor 1
NCBI Official Symbol:
CSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
P09603
Protein GI Number:
311033367
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Immunology,Signal Transduction
Swissprot #:
P09603
User NOte:
Optimal dilutions for each application to be determined by the researcher.