CRYGD Antibody
| Code | Size | Price |
|---|
| PSI-60-145-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Gamma-crystallin D, Gamma-D-crystallin, Gamma-crystallin 4, CRYGD, CRYG4
Application Note:
For WB starting dilution is: 1:1000
Background:
Crystallins are separated into two classes:
taxon-specific, or enzyme, and ubiquitous. The latter class
constitutes the major proteins of vertebrate eye lens and maintains
the transparency and refractive index of the lens. Since lens
central fiber cells lose their nuclei during development, these
crystallins are made and then retained throughout life, making them
extremely stable proteins. Mammalian lens crystallins are divided
into alpha, beta, and gamma families; beta and gamma crystallins
are also considered as a superfamily. Alpha and beta families are
further divided into acidic and basic groups. Seven protein regions
exist in crystallins: four homologous motifs, a connecting peptide,
and N- and C-terminal extensions. Gamma-crystallins are a
homogeneous group of highly symmetrical, monomeric proteins
typically lacking connecting peptides and terminal extensions. They
are differentially regulated after early development. Four
gamma-crystallin genes (gamma-A through gamma-D) and three
pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a
genomic segment as a gene cluster. Whether due to aging or
mutations in specific genes, gamma-crystallins have been involved
in cataract formation.
Background References:
- Acosta-Sampson, L., et al. J. Mol. Biol. 401(1):134-152(2010)
- Wang, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 107(30):13282-13287(2010)
- Pande, A., et al. Biochemistry 49(29):6122-6129(2010)
- Das, P., et al. Protein Sci. 19(1):131-140(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This CRYGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 73-101 amino acids from the Central region of human CRYGD.
NCBI Gene ID #:
1421
NCBI Official Name:
Gamma-crystallin D
NCBI Official Symbol:
CRYGD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P07320
Protein GI Number:
2506321
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P07320
User NOte:
Optimal dilutions for each application to be determined by the researcher.