POU3F3 Antibody

ProSci
Product Code: PSI-57-077
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-077-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CEM cell line lysates (35ug/lane).

Western blot analysis in CEM cell line lysates (35ug/lane).

Further Information

Additional Names:
POU domain, class 3, transcription factor 3, Brain-specific homeobox/POU domain protein 1, Brain-1, Brn-1, Octamer-binding protein 8, Oct-8, Octamer-binding transcription factor 8, OTF-8, POU3F3, BRN1, OTF8
Application Note:
For WB starting dilution is: 1:1000
Background:
POU3F3 is a member of the class III POU family of transcription factors (see POU3F1; MIM 602479) that are expressed in the central nervous system. The POU domain in these proteins is required for high affinity binding to octamer DNA sequences (summarized by Sumiyama et al., 1996 [PubMed 8703082]).[supplied by OMIM].
Background References:
  • Wissmuller, S., et al. Nucleic Acids Res. 34(6):1735-1744(2006)
  • Kuhlbrodt, K., et al. J. Biol. Chem. 273(26):16050-16057(1998)
  • Sumiyama, K., et al. Mamm. Genome 9(2):180-181(1998)
  • Malik, K.F., et al. Brain Res. Mol. Brain Res. 45(1):99-107(1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse, Rat
Immunogen:
This POU3F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 294-323 amino acids from the Central region of human POU3F3.
NCBI Gene ID #:
5455
NCBI Official Name:
POU domain, class 3, transcription factor 3
NCBI Official Symbol:
POU3F3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P20264
Protein GI Number:
2506534
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P20264
User NOte:
Optimal dilutions for each application to be determined by the researcher.