EIF3K Antibody

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ProSci
Product Code: PSI-58-722
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-722-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in T47D cell line lysates (35ug/lane).
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Western blot analysis in T47D cell line lysates (35ug/lane).

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Further Information

Additional Names:
Eukaryotic translation initiation factor 3 subunit K {ECO:0000255|HAMAP-Rule:MF_03010}, eIF3k {ECO:0000255|HAMAP-Rule:MF_03010}, Eukaryotic translation initiation factor 3 subunit 12 {ECO:0000255|HAMAP-Rule:MF_03010}, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25 {ECO:0000255|HAMAP-Rule:MF_03010}, eIF-3 p28, EIF3K {ECO:0000255|HAMAP-Rule:MF_03010}
Application Note:
For WB starting dilution is: 1:1000
Background:
The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12. eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).
Background References:
  • Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
  • Lin, Y.M., et al. J. Cell. Sci. 121 (PT 14), 2382-2393 (2008) :
  • Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
  • Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This EIF3K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human EIF3K.
NCBI Gene ID #:
27335
NCBI Official Name:
Eukaryotic translation initiation factor 3 subunit K {ECO:0000255|HAMAP-Rule:MF_03010}
NCBI Official Symbol:
EIF3K
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
25 kDa
Protein Accession #:
Q9UBQ5
Protein GI Number:
23396628
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q9UBQ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.