SOX3 Antibody

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ProSci
Product Code: PSI-56-211
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-211-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SOX3 antibody detected the SOX3 protein (arrow).
2 / 2
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the SOX3 antibody detected the SOX3 protein (arrow).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Transcription factor SOX-3, SOX3
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.
Background References:
  • Dateki, S., et al. J. Clin. Endocrinol. Metab. 95(8):4043-4047(2010)
  • Sato, Y., et al. Mol. Cell. Biochem. 337 (1-2), 267-275 (2010) :
  • Mojsin, M., et al. Biochem. J. 425(1):107-116(2010)
  • Bleyl, S.B., et al. Am. J. Med. Genet.143A (23), 2785-2795 (2007) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Zebrafish, Chicken, Mouse, Xenopus
Immunogen:
This SOX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human SOX3.
NCBI Gene ID #:
6658
NCBI Official Name:
Transcription factor SOX-3
NCBI Official Symbol:
SOX3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P41225
Protein GI Number:
48429228
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P41225
User NOte:
Optimal dilutions for each application to be determined by the researcher.