NPFF Antibody

ProSci
Product Code: PSI-61-857
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-857-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of NPFF Antibody in 293 cell line lysates (35ug/lane)
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Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of NPFF Antibody in 293 cell line lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Pro-FMRFamide-related neuropeptide FF, FMRFamide-related peptides, Neuropeptide SF, NPSF, Neuropeptide FF, NPFF, Neuropeptide AF, NPAF, NPFF
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
FMFRamide-related protein precursor plays a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. FMRFAL encodes a preproprotein which is cleaved to form two active peptides with similar function.
Background References:
  • Yang, H.Y., et al. Neuropeptides 42(1):1-18(2008)
  • Roumy, M., et al. J. Biol. Chem. 282(11):8332-8342(2007)
  • Anko, M.L., et al. J. Neurochem. 96(2):573-584(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This NPFF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the C-terminal region of human NPFF.
NCBI Gene ID #:
8620
NCBI Official Name:
Pro-FMRFamide-related neuropeptide FF
NCBI Official Symbol:
NPFF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
12 kDa
Protein Accession #:
O15130
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
O15130
User NOte:
Optimal dilutions for each application to be determined by the researcher.