DUSP6 Antibody

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ProSci
Product Code: PSI-63-476
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-476-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Confocal immunofluorescent analysis of DUSP6 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Antibody is used in Western blot to detect DUSP6 in HeLa cell lysate.
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Confocal immunofluorescent analysis of DUSP6 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Antibody is used in Western blot to detect DUSP6 in HeLa cell lysate.

Documents

Further Information

Additional Names:
Dual specificity protein phosphatase 6, Dual specificity protein phosphatase PYST1, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3, DUSP6, MKP3, PYST1
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
DUSP6 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP)kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Two transcript variants encoding different isoforms have been found for the DUSP6 gene.
Background References:
  • Mod. Pathol. 18 (8), 1034-1042 (2005)
  • J. Hum. Genet. 50 (4), 159-167 (2005)
  • J. Biol. Chem. 279 (40), 41882-41891 (2004)
  • Biochemistry 42 (51), 15197-15207 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This DUSP6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-89 amino acids from the Central region of human DUSP6.
NCBI Gene ID #:
1848
NCBI Official Name:
Dual specificity protein phosphatase 6
NCBI Official Symbol:
DUSP6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q16828
Protein GI Number:
108860971
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
Q16828
User NOte:
Optimal dilutions for each application to be determined by the researcher.