MEOX2 Antibody

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ProSci
Product Code: PSI-59-857
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-857-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Fluorescent image of NIH-3T3 cell stained with MEOX2 Antibody .NIH-3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEOX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MEOX2 immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
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Fluorescent image of NIH-3T3 cell stained with MEOX2 Antibody .NIH-3T3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEOX2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).MEOX2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Homeobox protein MOX-2, Growth arrest-specific homeobox, Mesenchyme homeobox 2, MEOX2, GAX, MOX2
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
This gene encodes a member of a subfamily of non-clustered, diverged, antennapedia-like homeobox-containing genes. The encoded protein may play a role in the regulation of vertebrate limb myogenesis. Mutations in the related mouse protein may be associated with craniofacial and/or skeletal abnormalities, in addition to neurovascular dysfunction observed in Alzheimer's disease.
Background References:
  • Rose, J. Phd, et al. Mol. Med. (2010) In press :
  • Ohshima, J., et al. Genes Chromosomes Cancer 48(12):1037-1050(2009)
  • Irelan, J.T., et al. PLoS ONE(4), E5067 (2009) :
  • Chen, Y., et al. Blood 111(3):1217-1226(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MEOX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-192 amino acids from the Central region of human MEOX2.
NCBI Gene ID #:
4223
NCBI Official Name:
Homeobox protein MOX-2
NCBI Official Symbol:
MEOX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P50222
Protein GI Number:
93141286
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P50222
User NOte:
Optimal dilutions for each application to be determined by the researcher.