HAND2 Antibody

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ProSci
Product Code: PSI-58-872
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-872-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Fluorescent confocal image of Hela cell stained with HAND2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis in K562 cell line lysates (35ug/lane).
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Fluorescent confocal image of Hela cell stained with HAND2 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly.
Western blot analysis in K562 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Heart- and neural crest derivatives-expressed protein 2, Class A basic helix-loop-helix protein 26, bHLHa26, Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2, dHAND, HAND2, BHLHA26, DHAND
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.
Background References:
  • Shen, L., et al. Chin. Med. J. 123(13):1623-1627(2010)
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Voth, H., et al. BMC Mol. Biol. 10, 28 (2009) :
  • Morikawa, Y., et al. Circ. Res. 103(12):1422-1429(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Rat, Xenopus
Immunogen:
This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2.
NCBI Gene ID #:
9464
NCBI Official Name:
Heart- and neural crest derivatives-expressed protein 2
NCBI Official Symbol:
HAND2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P61296
Protein GI Number:
47117699
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P61296
User NOte:
Optimal dilutions for each application to be determined by the researcher.