PPT2 Antibody

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ProSci
Product Code: PSI-57-783
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-783-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
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PPT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
PPT2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Lysosomal thioesterase PPT2, PPT-2, 312-, S-thioesterase G14, PPT2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a member of the palmitoyl-protein thioesterase family. The encoded glycosylated lysosomal protein has palmitoyl-CoA hydrolase activity in vitro, but does not hydrolyze palmitate from cysteine residues in proteins. Three transcript variants encoding different isoforms have been described for this gene, with one of the isoforms being inactive. [provided by RefSeq].
Background References:
  • Hancock, D.B., et al. Nat. Genet. 42(1):45-52(2010)
  • Barcellos, L.F., et al. PLoS Genet.(10), E1000696 (2009) :
  • McKinnon, E., et al. Diabetes Obes Metab 11 SUPPL 1, 92-100 (2009) :
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PPT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-212 amino acids from the Central region of human PPT2.
NCBI Gene ID #:
9374
NCBI Official Name:
Lysosomal thioesterase PPT2
NCBI Official Symbol:
PPT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q9UMR5
Protein GI Number:
296453016
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q9UMR5
User NOte:
Optimal dilutions for each application to be determined by the researcher.