HCN1 Antibody

ProSci
Product Code: PSI-57-572
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-572-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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HCN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela cell line lysates (35ug/lane).
HCN1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, Brain cyclic nucleotide-gated channel 1, BCNG-1, HCN1, BCNG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Hyperpolarization-activated cation channels of the HCN gene family, such as HCN1, contribute to spontaneous rhythmic activity in both heart and brain.
Background References:
  • Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
  • Laurin, N., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (1), 95-103 (2009) :
  • Woolcott, C.G., et al. Breast Cancer Res. 11 (1), R10 (2009) :
  • Laurin, N., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (5), 600-605 (2008) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This HCN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 860-889 amino acids from the C-terminal region of human HCN1.
NCBI Gene ID #:
348980
NCBI Official Name:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1
NCBI Official Symbol:
HCN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
O60741
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
O60741
User NOte:
Optimal dilutions for each application to be determined by the researcher.