PSA Antibody

ProSci
Product Code: PSI-62-326
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-326-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PSA antibody in MCF-7 cell line lysates (35ug/lane)
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PSA Antibody IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis of PSA antibody in MCF-7 cell line lysates (35ug/lane)
PSA Antibody IHC analysis in formalin fixed and paraffin embedded human Skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Puromycin-sensitive aminopeptidase, PSA, Cytosol alanyl aminopeptidase, AAP-S, NPEPPS, PSA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
PSA is the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.
Background References:
  • Thompson,M.W., Peptides 24 (9), 1359-1365 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PSA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 709-737 amino acids from the C-terminal region of human PSA.
NCBI Gene ID #:
9520
NCBI Official Name:
Puromycin-sensitive aminopeptidase
NCBI Official Symbol:
NPEPPS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
103 kDa
Protein Accession #:
P55786
Protein GI Number:
51704228
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P55786
User NOte:
Optimal dilutions for each application to be determined by the researcher.