EphA7 Antibody

ProSci
Product Code: PSI-63-048
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-048-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of EphA7 using rabbit polyclonal EphA7 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA7 gene (Lane 2).
2 / 2
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EphA7 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of EphA7 using rabbit polyclonal EphA7 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphA7 gene (Lane 2).
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EphA7 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Ephrin type-A receptor 7, EPH homology kinase 3, EHK-3, EPH-like kinase 11, EK11, hEK11, EPHA7, EHK3, HEK11
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
EphA7 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.[1]
Background References:
  • Wilkinson, D.G., Nat Rev Neurosci 2(3):155-164 (2001). Xu, Q., et al., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 355(1399):993-1002 (2000). Holder, N., et al., Development 126(10):2033-2044 (1999). Zhou, R., Pharmacol. Ther. 77(3):151-181 (1998). Fox, G.M., et al., Oncogene 10(5):897-905 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This EphA7 antibody is generated from rabbits immunized with recombinant human EphA7 protein.
NCBI Gene ID #:
2045
NCBI Official Name:
Ephrin type-A receptor 7
NCBI Official Symbol:
EPHA7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
112 kDa
Protein Accession #:
Q15375
Protein GI Number:
146345416
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
Q15375
User NOte:
Optimal dilutions for each application to be determined by the researcher.