CTSH Antibody

ProSci
Product Code: PSI-62-866
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-866-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CTSH Antibody in HepG2 cell line lysates (35ug/lane)
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CTSH Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of CTSH Antibody in HepG2 cell line lysates (35ug/lane)
CTSH Antibody IHC analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Pro-cathepsin H, Cathepsin H mini chain, Cathepsin H, Cathepsin H heavy chain, Cathepsin H light chain, CTSH, CPSB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The protein is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of its gene has been correlated with malignant progression of prostate tumors.
Background References:
  • Bunatova,K., Int. J. Biol. Markers 24 (1), 47-51 (2009)
  • Decock,J., Int. J. Biol. Markers 23 (3), 161-168 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CTSH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human CTSH.
NCBI Gene ID #:
1512
NCBI Official Name:
Pro-cathepsin H
NCBI Official Symbol:
CTSH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
37 kDa
Protein Accession #:
P09668
Protein GI Number:
288558851
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
P09668
User NOte:
Optimal dilutions for each application to be determined by the researcher.