ENTPD2 Antibody

Contact us
ProSci
Product Code: PSI-63-897
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-897-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
3 / 5
4 / 5
Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
5 / 5
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Ectonucleoside triphosphate diphosphohydrolase 2, NTPDase 2, 361-, CD39 antigen-like 1, Ecto-ATP diphosphohydrolase 2, Ecto-ATPDase 2, Ecto-ATPase 2, ENTPD2, CD39L1
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.
Background References:
  • Chiang,W.C. Biochemistry 47 (33), 8775-8785 (2008)
  • Javed,R. Biochemistry 46 (22), 6617-6627 (2007)
  • Mukasa,T.Biochemistry 44 (33), 11160-11170 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
NCBI Gene ID #:
954
NCBI Official Name:
Ectonucleoside triphosphate diphosphohydrolase 2
NCBI Official Symbol:
ENTPD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
Q9Y5L3
Protein GI Number:
18203633
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q9Y5L3
User NOte:
Optimal dilutions for each application to be determined by the researcher.