PAX8 Antibody

ProSci
Product Code: PSI-62-514
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-514-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis of PAX8 Antibody in HL-60 cell line lysates (35ug/lane)
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Flow cytometric analysis of 293 cells using PAX8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PAX8 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of 293 cells using PAX8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Paired box protein Pax-8, PAX8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this family contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes.
Background References:
  • Ramos,H.E., et.al., Thyroid 19 (1), 61-68 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8.
NCBI Gene ID #:
7849
NCBI Official Name:
Paired box protein Pax-8
NCBI Official Symbol:
PAX8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q06710
Protein GI Number:
215273928
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q06710
User NOte:
Optimal dilutions for each application to be determined by the researcher.