SCGN Antibody

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ProSci
Product Code: PSI-57-839
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-839-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of *SCGN using rabbit polyclonal *SCGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SCGN gene.
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SCGN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of *SCGN using rabbit polyclonal *SCGN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SCGN gene.
SCGN Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Secretagogin, SCGN, SECRET
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
SCGN is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. SCGN is thought to be involved in KCL-stimulated calcium flux and cell proliferation.
Background References:
  • Skovhus,K.V., Scand. J. Immunol. 64 (6), 639-645 (2006)
  • Zierhut,B., Biochem. Biophys. Res. Commun. 329 (4), 1193-1199 (2005)
  • Mungall,A.J., Nature 425 (6960), 805-811 (2003)
  • Zhan,X., Pituitary(4), 189-202 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SCGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-84 amino acids from the N-terminal region of human SCGN.
NCBI Gene ID #:
10590
NCBI Official Name:
Secretagogin
NCBI Official Symbol:
SCGN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
32 kDa
Protein Accession #:
O76038
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience
Swissprot #:
O76038
User NOte:
Optimal dilutions for each application to be determined by the researcher.