PAX5 Antibody
| Code | Size | Price |
|---|
| PSI-55-999-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes a member of the paired box (PAX) family
of transcription factors. The central feature of this gene family
is a novel, highly conserved DNA-binding motif, known as the paired
box. PAX proteins are important regulators in early development,
and alterations in the expression of their genes are thought to
contribute to neoplastic transformation. This gene encodes the
B-cell lineage specific activator protein that is expressed at
early, but not late stages of B-cell differentiation. Its
expression has also been detected in developing CNS and testis and
so the encoded protein may also play a role in neural development
and spermatogenesis. This gene is located at 9p13, which is
involved in t(9;14)(p13;q32) translocations recurring in small
lymphocytic lymphomas of the plasmacytoid subtype, and in derived
large-cell lymphomas. This translocation brings the potent E-mu
enhancer of the IgH gene into close proximity of the PAX5 promoter,
suggesting that the deregulation of transcription of this gene
contributes to the pathogenesis of these lymphomas. Alternatively
spliced transcript variants encoding different isoforms have been
described but their biological validity has not been determined.
Background References:
- Song, J., et al. Arch. Pathol. Lab. Med. 134(11):1702-1705(2010)
- Okamoto, R., et al. Haematologica 95(9):1481-1488(2010)
- Best, A., et al. Leuk. Res. 34(8):1098-1102(2010)
- Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PAX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the Central region of human PAX5.
NCBI Gene ID #:
5079
NCBI Official Name:
Paired box protein Pax-5
NCBI Official Symbol:
PAX5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q02548
Protein GI Number:
417449
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
Q02548
User NOte:
Optimal dilutions for each application to be determined by the researcher.