MPZ Antibody

ProSci
Product Code: PSI-63-552
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-552-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of MPZ Antibody in CEM cell line lysates (35ug/lane)
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Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of MPZ using rabbit polyclonal MPZ Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPZ gene.

Western blot analysis of MPZ Antibody in CEM cell line lysates (35ug/lane)
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of MPZ using rabbit polyclonal MPZ Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MPZ gene.

Further Information

Additional Names:
Myelin protein P0, Myelin peripheral protein, MPP, Myelin protein zero, MPZ
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
MPZ is a major structural protein of peripheral myelin. Creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae.
Background References:
  • Tachi,N., et.al., Brain Dev.(6), 560-565 (1984)
  • Hayasaka,K.,et.al., Biochem. Biophys. Res. Commun. 180 (2), 515-518 (1991)
  • Hayasaka,K., et.al., Genomics 17 (3), 755-758 (1993)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MPZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from the C-terminal region of human MPZ.
NCBI Gene ID #:
4359
NCBI Official Name:
Myelin protein P0
NCBI Official Symbol:
MPZ
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P25189
Protein GI Number:
127721
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P25189
User NOte:
Optimal dilutions for each application to be determined by the researcher.