TBX1 Antibody

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ProSci
Product Code: PSI-63-920
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-920-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
2 / 5
Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody at 1:1000 at each lane.
3 / 5
4 / 5
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody . A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.
5 / 5
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H. breast section using TBX1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis of lysate from HLVEC cell line, using TBX1 Antibody at 1:1000 at each lane.
Fluorescent confocal image of A2058 cell stained with TBX1 Antibody . A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX1 immunoreactivity is localized to nucleus significantly.
Formalin-fixed and paraffin-embedded human testis tissue reacted with TBX1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
T-box transcription factor TBX1, T-box protein 1, Testis-specific T-box protein, TBX1
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
TBX1 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Background References:
  • Fernando, R.I., et al. J. Clin. Invest. 120(2):533-544(2010)
  • Heike, C.L., et al. Birth Defects Res. PartClin. Mol. Teratol. 88(1):54-63(2010)
  • Beaujard, M.P., et al. EurMed Genet 52(5):321-327(2009)
  • Shalaby, A.A., et al. Mod. Pathol. 22(8):996-1005(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This TBX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human TBX1.
NCBI Gene ID #:
6899
NCBI Official Name:
T-box transcription factor TBX1
NCBI Official Symbol:
TBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
O43435
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
O43435
User NOte:
Optimal dilutions for each application to be determined by the researcher.