NMB Antibody

ProSci
Product Code: PSI-61-894
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-894-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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NMB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
NMB Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Neuromedin-B, Neuromedin-B-32, Neuromedin-B, NMB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
NMB stimulates smooth muscle contraction in a manner similar to that of bombesin.
Background References:
  • Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
  • Spalova, J., et al. Physiol Res 57 SUPPL 1, S39-S48 (2008)
  • Hoggard, N., et al. J. Mol. Endocrinol. 39(3):199-210(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This NMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-42 amino acids from the Central region of human NMB.
NCBI Gene ID #:
4828
NCBI Official Name:
Neuromedin-B
NCBI Official Symbol:
NMB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
13 kDa
Protein Accession #:
P08949
Protein GI Number:
281185514
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience
Swissprot #:
P08949
User NOte:
Optimal dilutions for each application to be determined by the researcher.