Metabotropic Glutamate Receptor 7 Antibody

ProSci
Product Code: PSI-58-462
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-462-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
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Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody for immunohistochemistry.

Western Blot at 1:1000 dilution + SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Metabotropic Glutamate Receptor 7 (GPRC1G) Antibody for immunohistochemistry.

Further Information

Additional Names:
Metabotropic glutamate receptor 7, mGluR7, GRM7, GPRC1G, MGLUR7
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternative splice variants of GRM8 have been described but their full-length nature has not been determined.
Background References:
  • Schulz, H.L., et al., Neurosci. Lett. 326(1):37-40 (2002).
  • Flor, P.J., et al., Neuropharmacology 36(2):153-159 (1997).
  • Makoff, A., et al., Brain Res. Mol. Brain Res. 40(1):165-170 (1996).
  • Scherer, S.W., et al., Genomics 31(2):230-233 (1996).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This Metabotropic Glutamate Receptor 7 (GPRC1G) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 857-887 amino acids from the C-terminal region of human Metabotropic Glutamate Receptor 7 (GPRC1G).
NCBI Gene ID #:
2917
NCBI Official Name:
Metabotropic glutamate receptor 7
NCBI Official Symbol:
GRM7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
Q14831
Protein GI Number:
2495078
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Growth Factor
Swissprot #:
Q14831
User NOte:
Optimal dilutions for each application to be determined by the researcher.