MCHR1 Antibody

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ProSci
Product Code: PSI-55-342
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-342-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
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MCHR1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in CEM,K562 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.
MCHR1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM,K562 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Melanin-concentrating hormone receptor 1, MCH receptor 1, MCH-R1, MCHR-1, G-protein coupled receptor 24, MCH-1R, MCH1R, MCHR, SLC-1, Somatostatin receptor-like protein, MCHR1, GPR24, SLC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene, a member of the G protein-coupled receptor family 1, is an integral plasma membrane protein which binds melanin-concentrating hormone. The encoded protein can inhibit cAMP accumulation and stimulate intracellular calcium flux, and is probably involved in the neuronal regulation of food consumption. Although structurally similar to somatostatin receptors, this protein does not seem to bind somatostatin.
Background References:
  • Miyamoto-Matsubara, M., et al. Ann. N. Y. Acad. Sci. 1200, 112-119 (2010) :
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
  • Miller, C.L., et al. Schizophr. Res. 113 (2-3), 259-267 (2009) :
  • Gavalas, N.G., et al. Exp. Dermatol. 18(5):454-463(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This MCHR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-422 amino acids from the C-terminal region of human MCHR1.
NCBI Gene ID #:
2847
NCBI Official Name:
Melanin-concentrating hormone receptor 1
NCBI Official Symbol:
MCHR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
46 kDa
Protein Accession #:
Q99705
Protein GI Number:
33860175
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Neuroscience,Growth Factor
Swissprot #:
Q99705
User NOte:
Optimal dilutions for each application to be determined by the researcher.