CRHR2 Antibody

ProSci
Product Code: PSI-62-463
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-463-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of CRHR2 Antibody in Hela cell line lysates (35ug/lane)
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Confocal immunofluorescent analysis of CRHR2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of CRHR2 Antibody in Hela cell line lysates (35ug/lane)
Confocal immunofluorescent analysis of CRHR2 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Corticotropin-releasing factor receptor 2, CRF-R-2, CRF-R2, CRFR-2, Corticotropin-releasing hormone receptor 2, CRH-R-2, CRH-R2, CRHR2, CRF2R, CRH2R
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
CRHR2 is a receptor for corticotropin releasing factor.It Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Background References:
  • Yerges,L.M., et.al., J. Bone Miner. Res. (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This CRHR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-272 amino acids from the Central region of human CRHR2.
NCBI Gene ID #:
1395
NCBI Official Name:
Corticotropin-releasing factor receptor 2
NCBI Official Symbol:
CRHR2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
48 kDa
Protein Accession #:
Q13324
Protein GI Number:
6226847
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Neuroscience,Growth Factor
Swissprot #:
Q13324
User NOte:
Optimal dilutions for each application to be determined by the researcher.